SPR (Biacore)

Surface Plasmon Resonance (SPR) Service for Receptor Binding Kinetics

Gifford Bioscience’s SPR assays are a label-free technique to measure the specificity, kinetics and affinity of the binding interaction between compound and target recombinant protein. SPR measurement of a compound’s binding kinetics profile guides the development of lead compounds that demonstrate maximal target engagement.

SPR (Biacore) can be used with biological and small molecule therapeutics. Binding parameters can help characterize drug leads to select higher affinity compounds for further chemistry exploration, or guide selection of drug candidates with the slowest dissociation rates to ensure maximal target engagement.

BioLayer Interferometry (BLI) provides an alternative label-free technique. It has more assay design flexibility than SPR but has more limited options for protein immobilization onto the sensor surface.

Our Areas of Expertise:

Antibodies, fAbs and ScFv fragments
Viral antigen and antibody detection
Cytokines and receptor tyrosine kinases (RTKs) – small molecules and biologics
Chromatin modification enzymes (epigenetics) – small molecules
Transcription factors & nuclear hormone receptors – small molecules
Protein complex formation and disruption – small molecules and biologics
Plasma biomarker and in vitro diagnostic assays

Assay Formats:

Direct binding affinities (kinetic: k_a, k_d, K_D; equilibrium: K_D)
Solution phase binding inhibition (IC₅₀ and K_i)
Concentration analysis and analyte quantitation in biological samples

The Principle of SPR 

Diagram demonstrating principal of surface plasmon resonance

Graph of association and dissociation in SPR

SPR (Biacore) measures the refractive index of the material within a ~100 nm layer immediately above the gold-coated biosensor surface
A target protein (receptor) is first immobilized / captured onto the biosensor surface
A test substance (ligand) is then flowed over the surface to bind to the receptor
As the ligand binds to the receptor, the refractive index increases in real-time – this allows the binding association rate (k_a) to be measured
The ligand is then washed off the receptor with buffer and the refractive index falls in real time – this allows the dissociation rate (k_d) to be measured
The kinetic binding affinity constant (K_D) is then calculated as k_d / k_a

Example Data
Protein – Protein Interactions

Kinetic affinity (K_D) and solution-phase competition (IC₅₀ and K_i) analysis

ACE2 and Spike RBD

Angiotensin converting enzyme 2 (ACE2) covalently coupled onto a CM5 sensor. SARS-CoV2 Spike RBD protein binds to ACE2 with a kinetic affinity K_D of 30 nM.

IGF2R and STC1

Insulin-like growth factor 2 receptor (IGF2R) covalently- immobilized onto a CM5 sensor. Stanniocalcin 1 (STC1) binds to IGF2R with a kinetic affinity K_D of 16.8 nM.

IGF2R and STC1 ± CREG

STC1 covalently- immobilized onto a CM5 sensor. Preincubating IGF2R (17 nM) with CREG (0 – 228 nM) inhibits IGF2R binding to STC1 with an IC₅₀ of 102 nM and K_i of 50.7 nM.

Antibody – Antigen Interactions (Quantitation in Biological Samples)

SARS-CoV2 spike RBD – anti-spike antibody binding (in buffer and 10% serum)

His-tagged SARS-CoV2 spike receptor binding domain (RBD) protein covalently coupled to CM5 sensor surface. Rabbit anti-spike binding responses in buffer (HBS-EP) or buffer + 10% serum generated antibody calibration curves. Lower limit of detection (LLD): 3.9 ng/mL; lower limit of quantitation (LLQ): 15.6 ng/mL.

Receptor – Ligand Interactions

ACE2 + AT2 – kinetic affinity and equilibrium binding analysis

ACE2 covalently immobilized onto a CM5 sensor. Angiotensin II (AT2; 1046.2 Da) binding responses to ACE2 were fitted to a single-site kinetic affinity model (left) to give a K_D of 12.8 nM. Fitting the same data to an equilibrium binding model (center) using the response amplitudes marked at ‘x’, plotted on a linear saturation binding model (right), gives a K_D (concentration at half-maximal response) of 26.7 nM.

Receptor – Small Molecule Interactions

Protac ligand binding to Von Hippel–Lindau (VHL) protein

Protac ligand MZ1 (1002.64 Da) binding to covalently-immobilized Von Hippel-Lindau (VHL) protein gives a saturable binding response of 7.0 RUs with a K_D of 178 nM. In contrast, the non-binding control, cis-MZ1 (1002.64 Da) gives a non-saturable, non-specific binding response with an estimated K_D > 240 µM.

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